diff --git a/data/resources/sounds/content_start.wav b/data/resources/sounds/content_start.wav
deleted file mode 100644
index 77f0f4344..000000000
Binary files a/data/resources/sounds/content_start.wav and /dev/null differ
diff --git a/src/core/fullscreenui.cpp b/src/core/fullscreenui.cpp
index bc3f34310..d1b858d0e 100644
--- a/src/core/fullscreenui.cpp
+++ b/src/core/fullscreenui.cpp
@@ -127,7 +127,6 @@ static constexpr std::string_view ABOUT_DIALOG_NAME = "##about_duckstation";
 const char* SFX_NAV_ACTIVATE = "sounds/nav_activate.wav";
 const char* SFX_NAV_BACK = "sounds/nav_back.wav";
 const char* SFX_NAV_MOVE = "sounds/nav_move.wav";
-const char* SFX_CONTENT_START = "sounds/content_start.wav";
 
 //////////////////////////////////////////////////////////////////////////
 // State
@@ -618,8 +617,6 @@ void FullscreenUI::DoStartPath(std::string path, std::string state, std::optiona
   if (GPUThread::HasGPUBackend())
     return;
 
-  EnqueueSoundEffect(SFX_CONTENT_START);
-
   // Stop running idle to prevent game list from being redrawn until we know if startup succeeded.
   GPUThread::SetRunIdleReason(GPUThread::RunIdleReason::FullscreenUIActive, false);
 
diff --git a/src/core/fullscreenui.h b/src/core/fullscreenui.h
index fe1c9cc69..3bb2e62ec 100644
--- a/src/core/fullscreenui.h
+++ b/src/core/fullscreenui.h
@@ -101,7 +101,6 @@ private:
 extern const char* SFX_NAV_ACTIVATE;
 extern const char* SFX_NAV_BACK;
 extern const char* SFX_NAV_MOVE;
-extern const char* SFX_CONTENT_START;
 
 } // namespace FullscreenUI